论文部分内容阅读
本研究采用２０ｍｇ／ｍＬ的石棉生理盐水溶液，给大鼠灌肺，分别在第二、四、六、八周处死。用改良的Ｇｏｍｍｏｒ结缔组织特殊染色法染色，在光学显微镜下观察肺组织的病理变化。测其支气管肺泡灌洗液（ＢＡＬＦ）上清中肿瘤坏死因子（ＴＮＦ）的生物活性。发现２周ＴＮＦ水平最高，８周有肺纤维化形成；ＢＡＬＦ的肺泡巨噬细胞（ＭＰ）培养上清中，４８ｈ为ＴＮＦ释放高峰；石棉可刺激ＭＰ释放ＴＮＦ；秋水仙碱可抑制ＭＰ释放ＴＮＦ。
In this study, 20 mg / mL asbestos physiological saline solution was used to infuse the lungs of rats, and were sacrificed in the second, fourth, sixth and eighth weeks respectively. Staining was performed with a modified Gommor connective tissue staining method and the pathological changes of the lung tissue were observed under a light microscope. The biological activity of tumor necrosis factor (TNF) in the supernatant of bronchoalveolar lavage fluid (BALF) was measured. TNF levels were found to be the highest at 2 weeks and pulmonary fibrosis was formed at 8 weeks. The supernatant of alveolar macrophage (MP) in BALF was the peak of TNF release at 48 hours. Asbestos could stimulate the release of TNF by MP. Colchicine inhibited the release of TNF by MP .