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目的 :建立测定阿昔洛韦血药浓度的方法。方法 :采用高效液相色谱法 (HPLC)于 2 5 4nm处测定阿昔洛韦的血药浓度。结果 :样品在C18柱上分析 ,流动相为甲醇∶冰醋酸 (99∶1) ,紫外检测波长 2 5 4nm ,最低检测浓度为 0 .0 2 2mg·L-1,线性范围 0 .0 2 2～ 4.40 0mg·L-1,阿昔洛韦的回收率大于 90 % ,日内和日间精密度的日内日间误差分别小于 10 %和 13 %。结论 :本法具有简便、灵敏、快速、准确的优点 ,符合临床药代动力学试验中生物样品分析的要求
Objective: To establish a method for determination of acyclovir plasma concentration. Methods: HPLC was used to determine the concentration of acyclovir at 254nm. Results: The sample was analyzed by C18 column. The mobile phase consisted of methanol: glacial acetic acid (99:1), UV detection wavelength of 254 nm, minimum detectable concentration of 0.222 mg · L-1 and linear range of 0.022 ~ 4.40 0 mg · L-1, the recoveries of acyclovir were more than 90%, and the intra-day and inter-day errors were less than 10% and 13% respectively. Conclusion: This method is simple, sensitive, rapid and accurate, in line with the requirements of biological samples in clinical pharmacokinetic test