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目的观察人类脂肪干细胞(human adipose-derived stem cells,hASCs)对慢性脑缺血大鼠认知功能障碍的影响。方法体外分离、培养及扩增hASCs;利用结扎双侧颈总动脉的方法制备慢性前脑缺血大鼠模型(two-vessel occlusion model,2VO模型),分别于造模后1d、1周、5周经尾静脉注射hASCs(2×10~6/ml)。造模5周后进行行为学实验,观察缺血后不同时间点应用hASCs对大鼠认知功能障碍的影响。结果模型组的游泳时间明显长于假手术组(P<0.01);造模后3周给予hASCs对模型组大鼠的认知功能障碍有改善作用(P<0.05),而造模后1d及1周组的结果无统计学意义(P>0.05)。结论静脉给予hASCs可改善慢性脑缺血大鼠的认知功能,且慢性期治疗效果较急性期好。
Objective To observe the effect of human adipose-derived stem cells (hASCs) on cognitive dysfunction in rats with chronic cerebral ischemia. Methods Two-vessel occlusion model (2VO model) was established by ligation of bilateral common carotid arteries in vitro. The rats were sacrificed at 1, 1, 5, Weekly tail vein injection of hASCs (2 × 10 ~ 6 / ml). After 5 weeks of modeling, behavioral experiments were performed to observe the effect of hASCs on cognitive dysfunction in rats at different time points after ischemia. Results The swimming time of the model group was significantly longer than that of the sham operation group (P <0.01). The hASCs were improved 3 weeks after model establishment (P <0.05), while the rats’ Results of the week group had no statistical significance (P> 0.05). Conclusion Intravenous administration of hASCs can improve the cognitive function of chronic cerebral ischemia rats, and the treatment of chronic phase is better than acute phase.