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初步探究猴头菌生长过程中基质内部碳源代谢情况,进一步了解了木质纤维素酶酶活和还原糖含量变化与猴头菌生长发育的关系,并测定猴头菌在生长的不同阶段基质内不同部位的木质纤维素酶酶活、还原糖含量和pH值。基质内不同部位木质纤维素酶酶活和还原糖含量变化趋势基本相同,但是不同部位间酶活大小和还原糖含量有明显差异,在越靠近袋口的部位表现出越低的酶活和较少的还原糖含量。漆酶在基质刚长满菌丝时和采收后酶活较高,在原基形成时,漆酶仅在靠近袋口部位出现一个活性高峰;羧甲基纤维素酶和半纤维素酶酶活在原基形成时出现一个活性小高峰,采收时酶活达到最高;还原糖在基质刚长满菌丝时含量较高,随后降低,采收前和采收时含量达高峰。基质内不同部位pH值变化较小,未对酶活产生影响。偏相关性分析显示还原糖含量与漆酶、羧甲基纤维素酶酶活呈中度相关,与半纤维素酶酶活不相关。基质内不同部位碳代谢有明显差异,可能与基质内菌丝体处于不同生理阶段和不同分化程度相关。木质纤维素酶可能通过调节还原糖含量对猴头菌生长发育产生影响,漆酶与原基形成有密切联系。
The preliminary study on the internal carbon source metabolism during the process of growth of Hericium erinaceus further understand the relationship between the changes of enzyme activity and reducing sugar content of lignocellulose and the growth and development of Hericium erinia and determine the growth of Hericium erinia in different stages of growth Different parts of the lignocellulase enzyme activity, reducing sugar content and pH value. The changes of lignocellulase activity and reducing sugar content in different parts of the substrate were basically the same, but there were significant differences in enzyme activity and reducing sugar content among the different parts. The lower the enzyme activity and the more enzyme activity Less reducing sugar content. Laccase showed a high enzyme activity when the substrate was freshly covered with mycelium and the enzyme activity was high after harvesting. When the primordia were formed, the laccase appeared an active peak only near the pocket site. Carboxymethylcellulase and hemicellulase enzyme activities When the primordium was formed, a small active peak appeared, and the enzyme activity reached the highest when harvested. The content of reducing sugar was higher when the substrate was full of mycelium, then decreased, and the content reached the peak before harvest and harvest. The pH value of different parts of the matrix changes little, not affecting the enzyme activity. Partial correlation analysis showed that the content of reducing sugar was moderately correlated with laccase and carboxymethylcellulase activities, but not with hemicellulase activity. Carbon metabolism in different parts of the stroma was significantly different, and may be related to different physiological stages and different degrees of differentiation mycelium within the matrix. Lignocellulose may affect the growth and development of Hericium erinaceus by regulating the content of reducing sugars. Laccase is closely related to the formation of primordium.