【摘 要】
：
As a universal pathogen leading to neonatal defects and transplant failure,human cytomegalovirus (HCMV) has strict species specificity and this has prevented the development of a suitable animal model for the pathogenesis study.The mechanism of cross-spec
【机 构】
：
Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500
论文部分内容阅读
As a universal pathogen leading to neonatal defects and transplant failure,human cytomegalovirus (HCMV) has strict species specificity and this has prevented the development of a suitable animal model for the pathogenesis study.The mechanism of cross-species barrier remains elusive and there are so far no non-human cell culture models that support HCMV replication.The Chinese tree shrew (Tupaia belangeri chinensis) is a small laboratory animal and evolutionary closely related with primates.We investigated the susceptibility of primary tree shrew dermis fibroblasts (TSDF) to HCMV infection.Infection with a GFP-expressing HCMV virus resulted in green fluorescence in infected cells with the expression of IE1,UL44 and pp28.The titers of cell-free viruses reached l03 PFU/mL at 96 hpi,compared to titers of 104 PFU/mL observed in primary human foreskin fibroblasts.Our results suggested that TSDF was semi-permissive for HCMV infection.The TSDF model could be further used to investigate key factors influencing cross-species multiplication of HCMV.
其他文献
Chikungunya virus (CHIKV) is an arbovirus transmitted by Aedes mosquitos in tropical and subtropical regions across the world.After decades of sporadic outbreaks,it re-emerged in Africa,Asia,India Ocean and America suddenly,causing major regional epidemic
Epstein-Barr virus (EBV) is an important human dsDNA virus,which has been shown to be associated with several malignancies including about 10％ of gastric carcinomas.How EBV enters an epithelial cell has been an interesting project for investigation.“Cell-
Ticks are important vectors for the transmission of pathogens including viruses.The viruses carried by ticks also known as tick-borne viruses (TBVs),contain a large group of viruses with diverse genetic properties and are concluded in two orders,nine fami
As specific pathogens of noctuid pests,including Spodoptera exigua,S.litura,Helicoverpa armigera,and Mythimna separata,ascoviruses are suitable for the development of bioinsecticides.In this study,the infectivity of Heliothis virescens ascovirus 3j (HvAV-
Severe fever with thrombocytopenia syndrome virus (SFTSV) is a highly pathogenic tick-borne bunyavirus that causes lethal infectious disease and severe fever with thrombocytopenia syndrome (SFTS) in humans.The molecular mechanisms and host cellular factor
While host proteins incorporated into virions during viral budding from infected cell are known to play essential roles in multiple process of the life cycle of progeny virus,these characteristics have been largely neglected in studies on rabies virus (RA
Soft rot is an economically significant disease in potato and one of the major threats to sustainable potato production.This study aimed at isolating lytic bacteriophages and evaluating methods for and the efficacy of applying phages to control potato sof
Dear Editor,rnEpstein-Barr virus (EBV,also termed human herpesvirus-4) was the first identified human tumor virus.Since its discovery in 1964,studies have shown that EBV infects over 90％ of all people by the time they are adults (Williams and Crawford 200
Cetacean morbillivirus (CeMV),the most relevant pathogen impacting the health and conservation of several already threatened cetacean populations worldwide (Van Bressem et al.2014),has shown in recent years an apparently increased tendency to cross “inter
Omsk hemorrhagic fever virus (OHFV) is a tick-borne flavivirus classified as a biosafety level-4 (BSL4) pathogen.Studies of OHFV are restricted to be conducted within BSL4 laboratories.Currently,no commercial vaccines or antiviral drugs are available agai