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目的应用伯氏疏螺旋体全菌蛋白首次皮内注射,再次腹腔注射加强免疫诱导Lewis大鼠类风湿关节炎(RA)模型并通过检测血清细胞因子探讨该模型发病机制。方法将雄性Lewis大鼠54只随机分为螺旋体低剂量组、螺旋体中剂量组、螺旋体高剂量组、完全弗氏佐剂组、不完全弗氏佐剂组及PBS组,每组9只。采用关节炎指数和组织病理学方法观察大鼠的发病程度和病理特点。采用双抗体夹心ELISA法检测血清RF、TNF-α、IL-1β、IL-6和IL-17含量。结果螺旋体高剂量组大鼠关节炎发病率为66.7%。病变关节病理显示关节滑膜组织增生,炎性细胞浸润,软骨及骨质侵蚀,呈典型的关节炎改变。螺旋体高剂量组与完全弗氏佐剂组相比关节炎指数、组织病理学评分及血清RF、TNF-α、IL-1β、IL-6和IL-17水平的差异无统计学意义(P>0.05)。螺旋体高剂量组与PBS组相比关节炎指数、组织病理学评分及血清RF、TNF-α、IL-1β、IL-6和IL-17水平明显升高(P<0.05)。结论伯氏疏螺旋体全菌蛋白成功诱导大鼠关节炎,与人类RA的发病及病理特点极为相似,是研究RA较理想的模型之一。
OBJECTIVE: To study the pathogenesis of rheumatoid arthritis (RA) induced by Lewis lung cancer in Lewis rats by intraperitoneal injection of the first intraperitoneal injection of Borrelia burgdorferi whole body protein. Methods Fifty-four male Lewis rats were randomly divided into low-dose spirochaete group, middle-dose spirochete group, high-dose spirochete group, complete Freund’s adjuvant group, incomplete Freund’s adjuvant group and PBS group. The arthritis index and histopathology were used to observe the incidence and pathological characteristics of rats. Serum levels of RF, TNF-α, IL-1β, IL-6 and IL-17 were measured by double antibody sandwich ELISA. Results The incidence of arthritis in spirochete high dose group was 66.7%. Pathological joint pathology showed synovial tissue hyperplasia, inflammatory cell infiltration, cartilage and bone erosion, showed a typical change in arthritis. The arthritis index, histopathological score, serum levels of RF, TNF-α, IL-1β, IL-6 and IL-17 in high-dose spirochete group and complete Freund’s adjuvant group were not significantly different (P> 0.05). Compared with PBS group, arthritis index, histopathological score and serum levels of RF, TNF-α, IL-1β, IL-6 and IL-17 in spirochete high dose group were significantly increased (P <0.05). Conclusion The whole body protein of Borrelia burgdorferi successfully induced arthritis in rats, which is closely related to the pathogenesis and pathological features of human RA. It is one of the most ideal models for studying RA.