目的:对三白草药材不同药用部位中的三白草酮含量进行分析比较。方法:采用Welch ultimate LP-C18色谱柱(4.6 mm×250 mm,5μm),流动相为乙腈-水(60∶40),流速1.0 m L·min-1,检测波长240 nm,柱温35℃。结果:三白草酮进样质量浓度在4.124~123.72μg·m L-1(r=0.999 8)质量浓度范围内线性关系良好,平均加样回收率(n=6)为100.4%,RSD为2.8%。10个不同产地样品中均为地上部分三白草酮的含量最高,根茎中的含量最低。结论:三白草不同药用部位中三白草酮的含量存在明显差异,确定药用部位对临床疗效及药材质量的稳定性具有重要意义。 Objective: To analyze and compare the content of trivalence in different medicinal parts of Siberian herbs. Methods: The mobile phase consisted of acetonitrile-water (60:40) with a flow rate of 1.0 mL · min-1 on a Welch ultimate LP-C18 column (4.6 mm × 250 mm, 5 μm) . Results: The calibration curve was linear over the range of 4.124 ~ 123.72μg · m L-1 (r = 0.999 8) with an average recovery of 100.4% (n = 6) and the RSD was 2.8%. Among the 10 samples from different areas, the content of trifolalidone was the highest in aerial parts and the lowest in rhizomes. Conclusion: There are significant differences in the content of tribenuron in different medicinal parts of Saururus chinensis. It is of great importance to determine the medicinal parts for the clinical efficacy and the stability of medicinal materials.