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δ-OAT基因编码的鸟氨酸-δ-氨基转移酶是以鸟氨酸为前体合成脯氨酸途径中的关键酶。采用基因枪法将拟南芥δ-OAT基因导入粳稻品种中作321,通过PCR及分子杂交分析确定目的基因已插入水稻染色体中并得到超量表达。抗盐抗旱检测结果表明,水稻在受到渗透胁迫时会大量积累脯氨酸,各种条件下转基因水稻积累的脯氨酸是对照的5～15倍;同等胁迫条件下转基因株系相对生长更快,苗与根的生物学产量都要高于对照,最后种子产量也显著高于对照,如在0.1mol/L NaCl胁迫下转基因株系相对产量提高了16％～41％,说明δ-OAT基因超量表达并积累脯氨酸在抗渗透胁迫中有着重要作用,通过转化δ-OAT基因可以获得抗盐抗旱的基因工程水稻。
The δ-OAT gene encodes ornithine-δ-aminotransferase is a key enzyme in the synthesis of proline pathway using ornithine as a precursor. The Arabidopsis thaliana δ-OAT gene was introduced into japonica rice by gene gun method. The PCR product and molecular hybridization analysis confirmed that the target gene was inserted into the rice chromosome and overexpressed. The results of salt-resistance and drought-resistance test showed that proline was abundantly accumulated in rice under osmotic stress, and proline accumulation in transgenic rice was 5-15 times higher than that of control under the same conditions. Relative growth of transgenic lines was faster under the same stress , The seedling and root biological yield were higher than the control, the final seed yield was also significantly higher than the control, such as 0.1mol / L NaCl stress relative yield of transgenic lines increased by 16% to 41%, indicating δ-OAT gene Overexpression and accumulation of proline plays an important role in osmotic stress resistance. Gene-resistant rice plants with salt tolerance and drought tolerance can be obtained by transforming δ-OAT gene.