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斑茅(Erianthus arundinaceus Retz)是甘蔗的近缘属植物,具有多种优良特性,是甘蔗育种的重要种质资源之一。本实验使用基因组原位杂交(GISH)技术分析了甘蔗-斑茅杂种及回交后代F1、BC1和BC2的染色体构成与传递行为。结果表明:在F1代,来自斑茅HN92-77的染色体数目介于28～30条,来自热带种Badila的染色体数目介于38～40条,体细胞染色体数为2n=68～70,基本符合n+n的染色体传递方式;在BC1和BC2代,来自斑茅的染色体数分别为22～28条和13～15条,来自甘蔗的染色体数分别是87～94条和98～101条,其体细胞染色体数2n分别为110～121条和112～115条,基本上分别符合2n+n和n+n的染色体传递方式。在所观察的渐渗系中,均存在有染色体丢失的现象,但未观察到染色体发生交换与重组。
Erianthus arundinaceus Retz, a relative genus of sugarcane, has many excellent characteristics and is one of the important germplasm resources for sugarcane breeding. In this study, the chromosome composition and transmission of F1, BC1 and BC2 in sugarcane-locust hybrids and backcross generations were analyzed using genomic in situ hybridization (GISH). The results showed that in F1 generation, the number of chromosomes from Houttuynia cordata HN92-77 ranged from 28 to 30, the number of chromosomes from tropical species Badila ranged from 38 to 40, and the number of somatic chromosomes was 2n = 68 to 70, which was basically consistent with n + n. In chromosomes BC1 and BC2, the chromosome numbers from M. dabryanus were 22-28 and 13-15, respectively. The chromosome numbers from sugarcane were 87-94 and 98-101, respectively The number of somatic chromosomes 2n was 110-121 and 112-115, respectively, basically consistent with the 2n + n and n + n chromosome transfer. In the observed introgression system, there is a phenomenon of chromosomal loss, but did not observe chromosome exchange and recombination.