生活饮用水水质卫生要求生活饮用水中不得含有病原微生物〔1〕,常规检测微生物指标为总大肠菌群、耐热大肠菌群等,这些指标作为检验肠道致病菌的指示菌,是生活饮用水、水源水进行卫生学评价的重要内容。目前检测水中总大肠菌群有3种方法,即多管发酵法(MTF)、滤膜法(MF)、酶底物法〔2〕(enzyme substrate technique)。多管发酵法需要3部分,即乳糖发酵试验、分离培养、证实试验,该方法步 Drinking water quality requirements of life Drinking water shall not contain pathogenic microorganisms [1], routine detection of microbial indicators of total coliforms, heat-resistant coliform bacteria, these indicators as indicators of intestinal pathogenic bacteria test is life Drinking water, water source hygiene evaluation important content. At present, there are three methods for the detection of total coliforms in water, namely MTF, MF and enzyme substrate methods. Multi-tube fermentation requires 3 parts, namely, lactose fermentation test, separation and culture, confirmed the test, the method step