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从蒺藜(Tribulus terristris L)茎和叶中提出醇溶皂甙后,再提取水溶性粗多糖,其单糖组成及其摩尔比为Ara.,Rha.,Xyl.,GalA.,Gal.,Glc.,Man.,6.0:2.1:1:3.6:3.4:7.7:2.9.粗多糖经分级与纯化得一均一级分H,其单糖组成及其摩尔比为Ara.,Rha,Xyl,GalA,Gal,Glc,1.6:2.4:0.1:3.5:1.3:1。H为酸性杂多糖,分子量约为10万。经果胶酶酶解、β-D-半乳糖苷酶酶解,高碘酸氧化、Smith降解、部分酸水解、甲基化、与GC及GC-MS分析等,表明H为α-D-GalA(1→4)与L-Rha(1→2)可能交替连接构成的主链,一些Rha.上带有分枝。
After the alcohol-soluble saponins were extracted from the stems and leaves of Tribulus terristris L, water-soluble polysaccharides were extracted and their monosaccharide composition and molar ratio were Ara., Rha., Xyl., GalA., Gal., Glc. ,Man.,6.0:2.1:1:3.6:3.4:7.7:2.9. The crude polysaccharide was fractionated and purified to obtain a homogenous fraction H. Its monosaccharide composition and its molar ratio were Ara.,Rha,Xyl,GalA,Gal. Glc, 1.6:2.4:0.1:3.5:1.3:1. H is an acidic heteropolysaccharide having a molecular weight of about 100,000. After pectinase digestion, β-D-galactosidase digestion, periodate oxidation, Smith degradation, partial acid hydrolysis, methylation, and GC and GC-MS analysis, indicating that H is α-D- GalA (1→4) and L-Rha (1→2) may be connected alternately to form a main chain, with some branches on Rha.