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目的对新疆传统发酵乳品中乳酸菌进行分离鉴定并检测其耐药性。方法利用传统形态学鉴定法和生化鉴定等方法对新疆发酵乳中乳酸菌进行鉴定,采用纸片扩散法对分离鉴定的菌进行耐药性分析。结果从新疆发酵乳品中共分离出8株乳酸菌,经鉴定分别为瑞士乳杆菌(Lactobacillus helveticus)、植物乳杆菌(Lactobacillus plantarum)、马乳酒样乳杆菌(Lactobacillus kefianofaciens)、乳酸乳球菌(Lactococcus lactis)、副干酪乳杆菌(Lactobacillus paracasei)、副干酪乳杆菌类坚韧亚种(Lactobacillus paracasei subsp.tolerans)、哈尔滨乳杆菌(Lactobacillus harbinensis)、希氏乳杆菌(Lactobacillus hilgardii),并且发现8株乳酸菌对万古霉素、庆大霉素、阿莫西林、多西环素、环丙沙星、阿奇霉素、头孢他啶、头孢孟多具有一定敏感性。结论新疆发酵乳品中以乳杆菌居多,对常见抗生素具有一定的敏感性。
Objective To isolate and identify lactic acid bacteria in Xinjiang traditional fermented dairy products and test their drug resistance. Methods The lactic acid bacteria in fermented milk of Xinjiang were identified by traditional morphological identification and biochemical identification methods. The drug resistance of the isolated and identified bacteria was analyzed by disk diffusion method. Results Eight strains of lactic acid bacteria were isolated from the fermented dairy products of Xinjiang and identified as Lactobacillus helveticus, Lactobacillus plantarum, Lactobacillus kefianofaciens, Lactococcus lactis, Lactobacillus paracasei, Lactobacillus paracasei subsp. Tolerans, Lactobacillus harbinensis, Lactobacillus hilgardii, and it was found that 8 strains of lactic acid bacteria were resistant to the eternal Gentamicin, gentamicin, amoxicillin, doxycycline, ciprofloxacin, azithromycin, ceftazidime, cefadoxime has a certain sensitivity. Conclusion Lactobacillus is the most fermented dairy product in Xinjiang, which has certain sensitivity to common antibiotics.