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本文研究用硼氢化钾溶液使硒(Ⅳ)还原成H_2Se挥发分离出来,用邻菲罗啉铁(Ⅲ)溶液吸收,以分光光度法测定被H_2Se还原生成的橙红色邻菲罗啉亚铁来测定硒的方法。 1.试剂和仪器: 硒标准溶液:将光谱纯硒溶于硝酸,加水配成1.00毫克/毫升硒的贮备液,使用时用0.1NH_2SO_4稀释成1.00微克/毫升硒的标准溶液。硼氢化钾溶液(3％):将硼氢化钾溶于0.5％KOH的水溶液中,滤去不溶物,贮于聚乙烯瓶中。硒化氢吸收液:在50毫升的醋酸-醋酸钠缓冲液(pH=4)中,加入75毫升0.2％邻非罗啉
In this paper, with the solution of potassium borohydride, the selenium (Ⅳ) is reduced to H_2Se and volatilized, and then absorbed by the solution of phenanthroline iron (Ⅲ). The reddish-phenanthroline ferrous reduced by H_2Se was measured by spectrophotometry Determination of selenium method. 1. Reagents and Instruments: Selenium standard solution: The pure spectral selenium dissolved in nitric acid, add water to make 1.00 mg / ml selenium stock solution, use 0.1NH_2SO_4 diluted to 1.00 micrograms / ml selenium standard solution. Potassium borohydride solution (3%): The potassium borohydride was dissolved in an aqueous solution of 0.5% KOH, the insoluble material was filtered off and stored in a polyethylene bottle. Hydrogen Selenide Absorbate: In 50 ml of acetic acid-sodium acetate buffer (pH = 4), 75 ml of 0.2% orthoporpholine