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目的:建立以液-质联用法测定人血浆中匹卡米隆浓度的方法。方法:色谱柱为Agilent C18,流动相为甲醇-水(85∶15),流速为0.2mL·min-1,柱温为20℃,进样量为10μL,内标为瑞格列奈;用于定量分析的离子反应分别为m/z207.0→m/z121.1(匹卡米隆)和m/z451.3→m/z379.2(瑞格列奈)。结果:匹卡米隆血药浓度在50～10000μg·L-1范围内线性关系良好(r=0.9991);方法回收率在99.14%～104.27%之间,日内和日间RSD分别为2.12%～5.94%、7.30%～8.44%。结论:本方法灵敏、准确,可用于匹卡米隆的血药浓度监测及其药动学研究。
Objective: To establish a method for the determination of Picamilone in human plasma by liquid chromatography - mass spectrometry. Methods: The column was Agilent C18 with a mobile phase of methanol-water (85:15), a flow rate of 0.2 mL · min-1, a column temperature of 20 ℃ and an injection volume of 10 μL with repaglinide The ion reactions for quantitative analysis were m / z 207.0 → m / z 121.1 (picamilone) and m / z 451.3 → m / z 379.2 (repaglinide), respectively. Results: The plasma concentration of Picamilone was linear in the range of 50 ~ 10000μg · L-1 (r = 0.9991). The recoveries were between 99.14% and 104.27%. The intra- and inter-day RSD were 2.12% 5.94%, 7.30% ~ 8.44%. Conclusion: The method is sensitive and accurate and can be used to monitor the plasma concentration of picamilon and its pharmacokinetics.