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目的 观察微电流 (low amperage direct current,LDC)与游离氯 (free chlorine,FC)对水中脊髓灰质炎 I型病毒 (PV1 )的协同灭活效果 .方法 用微电流 0 .4～ 1 .2 m A·cm-2 协同氯 0 .2～ 0 .3 mg· L-1 处理污染 PV1水样 ,比较作用前后灭活率 K值评价灭活效果 ,用 Berenbaum方法判断微电流与氯灭活病毒有无协同效应 ,用蚀斑形成试验 (PFUA)和病毒细胞酶联免疫试验 (VELCIA)检测感染性和抗原性变化 .结果 实验观察到微电流达到 0 .4 m A· cm-2 对水中PV1有弱灭活作用 ,电流密度达到 1 .2 m A· cm-2 与 0 .2mg· L-1氯有协同灭活效应 ,微电流 1 .2 m A· cm-2与氯 0 .3mg· L-1协同消毒 30 min,水中病毒减少 4.0 8个对数级 ,而单独用氯仅减少 1 .93个对数级 ;协同作用后病毒感染性灭活增强 ,而抗原性灭活不明显 .结论 微电流协同氯可提高低浓度氯灭活水中病毒的效果
Objective To observe the synergistic inactivation effect of low amperage direct current (LDC) and free chlorine (FC) on poliovirus type I virus (PV1) in water.Methods The microcurrent of 0.4 ~ 1.2 m A · cm-2 synergistic chlorine 0.2 ~ 0.3 mg · L-1 treatment of contaminated PV1 water samples were compared before and after the inactivation rate K value evaluation of inactivation effect, Berenbaum method to determine micro-current and chlorine inactivated virus have No synergistic effect was detected by plaque formation assay (PFUA) and viral cell enzyme-linked immunosorbent assay (VELCIA) .Results The results showed that the micro-current reached 0.4 m A · cm- Weak inactivation, the current density reached 1.2 m A · cm-2 and 0.2 mg · L-1 chlorine synergistic inactivation effect, micro-current 1.2 m A · cm-2 and chlorine 0.3 mg · L -1 synergistic disinfection for 30 min, the waterborne virus decreased by 4.08 logarithm, while chlorine alone decreased by only 1.93 logarithm; synergistic viral inactivation was enhanced, while antigenic inactivation was not significant. Micro-current synergistic chlorine can enhance the effect of low concentration chlorine inactivating virus in water