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目的:通过Box-Behnken响应面法优化高压蒸法炮制何首乌工艺,并与传统炮制工艺进行比较.方法:以蒸制温度、蒸制时间和干燥温度作为考察因素,以多糖含量、二苯乙烯苷含量和两指标归一化值作为评价指标,采用Box-Behnken响应面法考察了各个因素对高压法炮制工艺何首乌中多糖和二苯乙烯苷含量的影响,并比较高压蒸法炮制品与传统炮制品中多糖和二苯乙烯苷含量的差异.结果:高压蒸法炮制何首乌的最佳工艺为:蒸制温度为125.4℃、蒸制时间为3.1 h、干燥温度为52℃.优化的高压蒸法炮制的何首乌中多糖和二苯乙烯苷含量分别为传统炮制法的1.24和5.26倍.结论:利用Box-Behnken响应面法优化高压法炮制何首乌,方法简便,预测性良好.“,”Objective:To optimize the high pressure steaming processing technology for Polygonum multiflorum Thunb.by Box-Behnken response surface methodology , and compare with the traditional processing .Methods:The effects of factors such as steaming temperature, steaming time and drying temperature on polysaccharide content , stilbene glucoside content and normalized value in Po-lygonum multiflorumThunb.were studied by Box-Behnken response surface methodology .The content differences of polysaccharides and stilbene glucoside between the high pressure steaming processed product and the traditional processed product were evaluated .Re-sul ts:The best high pressure steaming processing conditions for Poyl gonumm luitlfo urm Thunb .were as follows:the steaming tempera -ture was 1254.℃ , the steaming time was3.1 h, and the drying temperature was 52℃.The contents of polysaccharides and stilbene glycosides in Polygonum multiflorum Thunb.processed by the high pressure steaming method were 1.24-fold and 5.26-fold higher than those processed by the traditional method .Conclusion:Box-Behnken response surface method can be used to optimize the high pres-sure steaming processing for Polygonum multiflorum Thunb., and the method is simple and predictable .