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用薄荷伪造桥虫核型多角体病毒(Argroyamma agnata NPV)(以下简称Aa NPV)在室内感染斜纹夜蛾(Prodenia litura)幼虫,从死虫体内分离到一种NPV。经电镜观察,多角体蛋白分析、病毒核酸的限制性内切酶酶解分析等研究,证明此多角体直径为1.5—2.6/μm,病毒粒子为杆状,其大小为100—150×420nm,病毒粒子为多粒包埋型。提纯的多角体蛋白只有一种多肽,分子量为33,500d,提纯的病毒粒子的结构多肽至少有15种,其分子量范围为15,600—78,300d。病毒核酸为双股DNA,经EcoRI、EcoRI+BamHI酶解可相应得23条和28条DNA片段,根据酶谱计算测得该病毒DNA的平均分子量为74.09×10~6d,DNA的T_m=75.4℃,G+C含量为52.46%。将此病毒与薄荷伪造桥虫NPV、斜纹夜蛾NPV部分特性进行比较,发现该病毒与薄荷伪造桥虫NPV截然不同,而与斜纹夜蛾NPV很相似,这种现象可能是潜伏感染所致。
An NPV was isolated from dead insects by infecting the larvae of Prodenia litura indoors with peppermint fake Argroyamma agnata NPV (Aa NPV). The results of electron microscopy, polyhedrin analysis and restriction endonuclease analysis of viral nucleic acid showed that the polyhedron was 1.5-2.6 / μm in diameter and the particle size was 100-150 × 420 nm. Virus particles are multi-embedding. The purified polyhedrin has only one polypeptide with a molecular weight of 33,500d. The purified virions have at least 15 structural polypeptides with molecular weights in the range of 15,600-78,300d. The viral nucleic acid was double-stranded DNA. After enzymatic digestion with EcoRI and EcoRI + BamHI, 23 and 28 DNA fragments were obtained. The average molecular weight of the virus DNA was 74.09 × 10 ~ 6d and the Tm of DNA was 75.4 ℃, the content of G + C is 52.46%. The comparison of this virus with the characteristics of NPV and Spodoptera litura NPV of peppermint false bridge found that the virus is quite different from NPV of counterfeit bridged moth and similar to NPV of Spodoptera litura, which may be caused by latent infection.