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目的:明确北青龙衣中萘醌类有效成分动态积累过程,确定北青龙衣药材的适宜采收期。方法:以不同采样方式获得多产区不同采集时期北青龙衣样本,采用紫外分光光度法测定总萘醌含量,采用高效液相色谱法测定胡桃醌、胡桃酮含量,结合药材产量,研究萘醌类成分的动态积累规律,确定适宜采收时期。结果:3个产区各采集时期北青龙衣样本的总萘醌、胡桃醌、胡桃酮含量整体呈逐渐下降趋势,8月中旬后急剧下降。结合药材产量,7月中旬至8月初有效成分总量积累达到最大值。结论:综合总萘醌、胡桃醌、胡桃酮含量以及药材产量确定北青龙衣适宜采收时间为7月中旬至8月初。
OBJECTIVE: To clarify the dynamic accumulation process of naphthoquinones in Beilong drapery, and to determine the suitable harvesting period of Beilong drape herbs. Methods: The sample of Beiqing Longyi with different sampling time was obtained by different sampling methods. The content of total naphthoquinone was determined by UV spectrophotometry. The content of juglone and juglone was determined by high performance liquid chromatography, Dynamic accumulation of components of the law to determine the appropriate harvest time. Results: The content of total naphthoquinone, juglone and kenaf ketone in the samples of Beiqing Longyi decreased gradually throughout the three producing areas and dropped sharply after mid-August. Combined with the production of medicinal herbs, the total amount of active ingredients accumulated reaches the maximum from mid-July to early August. Conclusion: According to the content of total naphthoquinone, juglone and kaempferol and the yield of medicinal herbs, the suitable harvesting time of Beilong Qingyi is from mid July to early August.