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目的研究抗凋亡蛋白BRE、TNFR1蛋白和自体吞噬蛋白Beclin1在小鼠肝纤维化的表达,并观察Reversine对其表达的影响。方法 24只昆明小鼠随机分为6组:第1组不作任何处理;第2组皮下注射0.1 mLCCl4/oil2,4 h后处死取肝组织;第3组皮下注射0.1 mL CCl4/oil4,8 h后处死;第4组皮下注射0.1 mL CCl4/oil,每周2次,连续注射6周,停止用药后15 d处死;第56、组方法同第4组,停止注射CCl4后分别用Reversine 501、00mg/(kg.d)皮下注射15 d,15 d后进行免疫组织化学检测,对BRE、TNFR1和Beclin1 3种蛋白的表达进行分析。结果免疫组化结果发现BRE、TNFR1和Beclin1 3种蛋白在CCl4诱肝纤维化过程中的表达明显上升,而经过纤维化抑制剂Reversine的处理后3种蛋白的表达下降至正常小鼠肝的水平。结论 BRE、TNFR1和Beclin1 3种应激反应分子可能在肝纤维化与肝炎发病中起重要作用,它们有可能成为肝病诊断标志物和治疗肝脏疾病的重要靶点。
Objective To study the expression of anti-apoptotic protein BRE, TNFR1 protein and autophagy protein Beclin1 in mouse liver fibrosis and to observe the effect of Reversine on their expression. Methods Twenty-four Kunming mice were randomly divided into 6 groups: group 1 received no treatment; group 2 injected subcutaneously with 0.1 m LCCl 4 / oil 2 for 4 h and sacrificed liver tissue; group 3 injected subcutaneous 0.1 mL CCl 4 / oil 4, 8 h The rats in group 4 were injected subcutaneously with 0.1 mL of CCl4 / oil twice a week for 6 weeks, and were sacrificed 15 days after stopping the treatment. Group 56 was given the same method as in group 4, and CCl4 was stopped after injection. Reversine 501, Immunohistochemistry was used to detect the expression of BRE, TNFR1 and Beclin1 after 15 days and 15 days after subcutaneous injection at 00mg / (kg · d) respectively. Results The results of immunohistochemistry showed that the expressions of BRE, TNFR1 and Beclin1 protein in CCl4-induced liver fibrosis increased significantly, while the expression of three proteins decreased after treatment with Reversine, a fibrosis inhibitor, to normal liver . Conclusion Three stress response molecules, BRE, TNFR1 and Beclin1, may play an important role in the pathogenesis of liver fibrosis and hepatitis, which may become a diagnostic marker of liver disease and an important target for the treatment of liver diseases.