采用光谱吸收法,对一株分离自土壤的嗜铁细菌洋葱伯克霍尔德式菌Burkholderia cepacia CAS19产嗜铁素的条件进行研究。结果表明,MSA培养基最适于CAS19产嗜铁素,并且在320nm处有一个嗜铁素特异吸收峰;当铁离子浓度大于1μmol/L时,几乎完全抑制CAS19嗜铁素的产生;CAS19产嗜铁素的最适条件为:以蔗糖(sucrose)为C源,以脯氨酸(Pro)为N源,C/N比为10∶1,pH值为7.5,温度为30℃,通气量为20mL/250mL。 Spectral absorption was used to study the condition of producing an effusive ferritin from Burkholderia cepacia CAS19, an iron bacterium isolated from soil. The results showed that MSA was the most suitable for the production of CAS19 and had a specific absorption peak at 320 nm. When the concentration of iron was more than 1 μmol / L, the production of CAS19 was almost completely inhibited. The optimal conditions for adding ferritin were: sucrose as C source, Pro as N source, C / N ratio of 10: 1, pH value of 7.5, temperature of 30 ℃, aeration 20 mL / 250 mL.