论文部分内容阅读
OBJECTIVE:To explore the effects of Taohong Siwu decoction(桃红四物汤,THSWD)on the extracellu-lar matrix of endometrium in rats following drug-in-duced abortion.METHODS:Thirty-six pregnant female rats were administered mifepristone and misoprostol to in-duce abortion,and amounts of uterine bleeding were recorded.Pathological damage and collagen accumulation were detected by hematoxylin-eo-sin staining and Masson's trichrome staining in uterus,respectively.Myeloperoxidase was evaluat-ed by immunohistochemistry.The expression lev-els of fibronectin,laminin,matrix metalloprotein-ase 9(MMP-9),and tissue inhibitor of metallopro-teinase 1(TIMP-1)were quantified using western blotting.RESULTS:THSWD could promote endometrial pro-tection in rats following drug-induced abortion.The contents of cellulose and myeloperoxidase were significantly decreased in uterine tissue of THSWD-treated groups.Moreover,THSWD signifi-cantly decreased the expression levels of fibronec-tin,laminin,and TIMP-1.THSWD also significantly increased MMP-9 expression and the MMP-9/TIMP-1 ratio.CONCLUSION:THSWD plays a critical role in en-dometrial protection by reducing extracellular ma-trix deposition and uterine fibrosis.These effects may have been achieved by increasing MMP-9,re-ducing TIMP-1,and/or altering the ratio of MMP-9/TIMP-1.