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双酶偶联体系越来越受到人们重视,广泛应用于医药、食品等领域,是实现高效生物合成的重要方法。本文利用乳酸氧化酶(Lactate Oxidase,LOD)和丙酮酸脱羧酶(Pyruvatedecarboxylase,PDC)偶联构建双酶体系,催化乳酸钠和苯甲醛合成L-苯基乙酰基甲醇(L-Phenylacetylcarbinol,L-PAC),后者是合成L-麻黄素的重要中间体。转化反应的最佳温度为34℃,pH 6.8,转化时间6 h,苯甲醛用量160 mmol/L,乳酸钠用量330 mmol/L,葡萄糖用量3 g/100 mL,L-PAC产量达到8.79 g/L。在L-PAC的制备过程中,以廉价的乳酸钠代替丙酮酸大大降低了生产成本,缩短了反应时间,简化了操作步骤,有利于工业生产。双酶偶联体系为生物合成L-PAC提供了一个新的思路和新方法。
Enzyme coupling system is getting more and more attention. It is widely used in medicine, food and other fields and is an important method to achieve efficient biosynthesis. In this paper, L-phenylacetylcarbinol (L-PAC) was synthesized by coupling Lactate Oxidase (LOD) and Pyruvatedecarboxylase (PDC) , Which is an important intermediate for the synthesis of L-ephedrine. The optimum reaction temperature was 34 ℃, pH 6.8, transformation time 6 h, the dosage of benzaldehyde 160 mmol / L, the dosage of sodium lactate 330 mmol / L, the dosage of glucose 3 g / 100 mL, the yield of L-PAC 8.79 g / L . In the process of preparation of L-PAC, cheap sodium lactate instead of pyruvic acid greatly reduces the production cost, shortens the reaction time, simplifies the operation steps and is good for industrial production. Double enzyme coupling system provides a new idea and new method for biosynthesis of L-PAC.