军团菌是引起非典型肺炎的重要病原体之一.针对目前军团菌临床检测时间长,特异性、敏感度不够等问题,该文运用抗原和抗体特异性结合的原理,根据军团菌糖蛋白的化学结构,获得军团菌抗原,并通过杂交瘤技术制备单克隆抗体,筛选得到亲和力高的杂交瘤细胞株;为开发军团菌检测试剂盒奠定基础. Legionella is one of the important pathogens causing SARS.According to the current clinical detection of Legionella in a long time, specificity, lack of sensitivity and other issues, the use of antigen and antibody specific binding principle, according to the Legionella glycoprotein chemistry Structure, obtain Legionella antigens, and prepare monoclonal antibodies by the hybridoma technology, and screen the hybridoma cell strains with high affinity; and lay a foundation for the development of a test kit for Legionella bacteria.