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目的:探讨在小鼠增龄过程中胸腺细胞退行性改变的机制。方法:选用20只不同月龄的昆明小鼠,共分为4组(乳鼠组、1月龄鼠组、3月龄鼠组及6月龄鼠组),用颈椎脱臼法处死小鼠,取出胸腺,通过HE染色检测各月龄小鼠胸腺细胞的状态和分布变化;采用流式细胞仪检测各月龄小鼠胸腺细胞的自然凋亡率及细胞周期各时相的细胞比例。结果:6月龄鼠组小鼠胸腺严重萎缩,胸腺细胞自然凋亡率与其他组比较,差异具有统计学意义。各组小鼠不同细胞周期的胰腺细胞分布不同,S期细胞凋亡速度最快。结论:小鼠增龄相关的退行性改变可能主要作用于S期进入G_2期的相关蛋白。
Objective: To investigate the mechanism of thymocyte degeneration in the mouse aging. Methods: Twenty Kunming mice of different ages were selected and divided into 4 groups (suckling group, 1-month-old mouse group, 3-month-old mouse group and 6-month old mouse group), mice were killed by cervical dislocation, The thymus was removed and the thymocytes were examined by HE staining to examine the changes of the status and distribution of thymocytes in each age mouse. The apoptotic rates of thymocytes and the percentage of cells in each phase of the cell cycle were measured by flow cytometry. Results: The thymus of mice in 6-month-old mice was severely atrophied. Compared with other groups, the apoptotic rate of thymus cells was statistically significant. The distribution of pancreatic cells in different cell cycle in different groups was different, and the apoptosis rate in S phase was the fastest. Conclusion: The age-related degenerative changes in mice may mainly play an important role in the phase 2 S phase G 2 proteins.