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应用核基因组SSR和叶绿体基因组SSR,分析了枳属28份枳及枳杂种种质的遗传多样性及其与近缘属植物的亲缘关系。核基因组SSR分析表明普通枳的遗传差异明显,22个SSR位点的平均多态性信息含量(PIC)为0.51,平均期望杂合度为0.52,表明中国枳种质资源具有较丰富的遗传多样性。在遗传距离约0.16时,22份普通枳可以分成4个类型。叶绿体基因组SSR分析则发现普通枳间基本无差异,表明以母系遗传为特征的枳叶绿体基因组相对保守。富民枳与普通枳无论是在核基因组还是在叶绿体基因组上均存在较大差异,支持富民枳种的地位。SSR和cpSSR结合使用可比较准确地鉴定枳杂种。
Using the nuclear genome SSR and the chloroplast genome SSR, the genetic diversity of 28 hybrids of trifoliate orange and trifoliate orange (Poncirus trifoliata) and its related genera to relatives was analyzed. The SSR analysis of nuclear genome showed that the genetic diversity of common Trifoliate orange was obvious. The PIC of the 22 SSR loci was 0.51, and the average expected heterozygosity was 0.52, which indicated that the genetic diversity of Trifoliate Orange germplasm was richer . At the genetic distance of about 0.16, 22 common trifoliate oranges can be divided into four types. The chloroplast genome SSR analysis found that there was no difference among the common trifoliate oranges, indicating that the trifoliate orange chloroplast genome is characterized by maternal inheritance. Fumin Trifoliate and Trifoliate Orange both in the nuclear genome or in the chloroplast genome are quite different, support Fumin Zhizu species status. The combination of SSR and cpSSR can identify the trifoliate hybrids more accurately.