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目的探讨在沉降法测定空气中菌落总数时,用一次性塑料平皿和玻璃平皿对监测结果的影响。方法在医院Ⅲ类区及公共场所各设25个采样点,同一采样点、同一时间、同时放置两种平皿采用自然沉降法采集空气中的菌落总数进行监测结果比较;并用倾注法验证日常菌种接种中使用玻璃平皿与一次性塑料平皿的差异性。结果在医院Ⅲ类区及公共场所各设的25个采样点中,医院Ⅲ类区用玻璃平皿收集培养的结果平均为33,用一次性塑料平皿收集培养的结果平均为27;公共场所用玻璃平皿收集培养的结果平均为14,用一次性塑料平皿收集培养的结果平均为11。结论使用不同材质的平皿对沉降法测定空气中菌落总数结果有显著的影响,用玻璃平皿收集培养的空气中的菌落总数多于用一次性塑料平皿收集培养的空气中的菌落总数,沉降法测定空气菌落总数监测中建议应用玻璃平皿。
Objective To investigate the effect of disposable plastic plate and glass plate on the monitoring results when determining the total number of colonies in air by sedimentation method. Methods Twenty-five sampling points, the same sampling point, the same time and two types of dishes were collected in the type Ⅲ area of public hospital and public places respectively. The total number of colonies in the air was collected by natural sedimentation method to compare the monitoring results. Differences between glass plates and single-use plastic plates for inoculation. Results Among the 25 samples collected from hospitals in category Ⅲ and public places, the average number of samples collected and collected by the glass plates in the hospital Ⅲ area was 33, and the average of the results collected and collected in disposable plastic dishes was 27. Glass for public places The results of plate collection and culture were 14 on average, and the results of collection and culture in disposable plastic dishes averaged 11. Conclusion The sedimentation method was used to determine the total number of colonies in the air. The total number of colonies in the air collected by the glass plate was more than the total number of colonies in the air collected by the disposable plastic plate. The total number of air colonies monitoring the application of glass dishes.