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目的 研究细胞角蛋白 2 0 (CK2 0 )和癌胚抗原 (CEA)单抗免疫细胞化学 (ICC)染色方法在胃癌病人腹腔冲洗液脱落癌细胞检测中的临床应用价值。方法 应用CK2 0和CEA两种标志物 ,对 30例胃癌病人腹腔冲洗液细胞涂片进行免疫细胞化学染色及常规腹腔冲洗液细胞学检查 (CY) ,检测腹腔内脱落癌细胞。结果 ICC阳性检出率 (43 3%)明显高于CY (2 3 3%) (P <0 0 5 ) ;CK2 0单抗检出 13例ICC阳性病例中的 12例(92 3%) ,优于CEA(76 9%) ;腹腔内癌细胞的脱落与浆膜受侵、胃癌分化程度、有无淋巴结转移相关 (P <0 0 5 ) ,并与临床分期密切相关 (P <0 0 1)。结论 采用CK2 0单抗免疫细胞化学染色方法检测腹腔冲洗液中脱落癌细胞是一种简单有效的方法 ,有临床实用价值。
Objective To investigate the clinical value of cytokeratin 20 (CK20) and immunocytochemistry (ICC) staining of carcinoembryonic antigen (CEA) monoclonal antibody in the detection of exfoliative cancer cells in patients with gastric cancer. Methods CK20 and CEA markers were used to perform immunocytochemical staining and routine abdominal lavage fluid cytology (CY) cytology for peritoneal washing fluid cell smears in 30 patients with gastric cancer to detect peritoneal exfoliative cancer cells. Results The positive rate of ICC (43 3%) was significantly higher than that of CY (2.33%) (P <0 05). The CK20 monoclonal antibody detected 12 cases (92 3%) of the 13 ICC positive cases. It was better than CEA (76 9%). The shedding of intra-abdominal cancer cells was related to serosal invasion, gastric cancer differentiation, and lymph node metastasis (P < 0.05), and it was closely related to clinical stage (P <0 0 1 ). Conclusion CK20 monoclonal antibody immunocytochemistry staining method is a simple and effective method for the detection of exfoliative cancer cells in peritoneal lavage fluid. It has clinical value.