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莲瓣兰是我国珍贵的兰花野生资源,具有很高的观赏价值,然而其相关的分子生物学研究尚属空白。为揭示莲瓣兰花朵形成的分子机制,应用高通量测序技术对莲瓣兰花朵进行转录组分析。原始数据经过生物信息学分析后,共获得57 011条Unigene;所获得的Unigene与Nr,Swiss-prot,COG和KEGG等数据库进行搜索比对后,发现Unigene的注释总量为24 049条,约32 962条Unigene没获得注释,这些没获得注释Unigene基因可被认为是莲瓣兰新的转录本和特异基因。36条CDS基因被注释为MADS-box基因,构建系统进化树后并对31条MADS-box基因进行了聚类,其中23条为与花型发育相关的ABCDE类基因,8条为与开花时间相关的MADS基因,同时发现PI、AP3和AGL6基因表达量较高,该试验为研究兰属的花发育以及开花机理的研究提供一定的理论依据。
Lilac orchid is a precious orchid resource in China, which has a high ornamental value. However, its related molecular biology research is still blank. To reveal the molecular mechanism of orchid flower formation, transcriptome analysis was performed using high-throughput sequencing technique. After the original data were analyzed by bioinformatics, a total of 57 011 Unigene were obtained. After Unigene was searched and compared with databases such as Nr, Swiss-prot, COG and KEGG, it was found that there were 24 049 Unigene annotations 32 962 Unigene No comment, no comment on these Unigene The gene can be considered as a new transcript and a specific gene of Cymbidium. 36 CDS genes were annotated as MADS-box genes. After phylogenetic tree was constructed, 31 MADS-box genes were clustered. Among them, 23 were ABCDE genes related to floral development and 8 were related to flowering time Related MADS genes, and found that the expression of PI, AP3 and AGL6 genes are high. This experiment provides a theoretical basis for the study on flower development and flowering mechanism of Cymbidium.