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目的研究灯盏花素(Bre)、顺铂(Cis)以及两药联用对小鼠黑色素瘤的作用及其机制。方法用C57小鼠建立皮下移植瘤模型,小鼠分为4组,对照组(注射二甲基亚砜),Bre组(注射4μg·g~(-1)Bre),Cis组(注射5μg·g~(-1)Cis),Bre+Cis组(注射Bre与Cis),隔天注射,共计15 d,每次治疗后测量肿瘤体积。用免疫组织化学方法检测Brd U及CD31表达,流式细胞术检测肿瘤周期。用3-(4,5-二甲基噻唑-2)-2,5-二苯基四氮唑溴盐(MTT)法检测小鼠黑色素瘤细胞增殖。结果与对照组相比,Bre组、Cis组以及Bre+Cis组均能明显减小肿瘤体积和肿瘤重量(P<0.05,或P<0.01);同时,3组均能明显抑制黑色素瘤细胞的增殖与血管新生(P<0.05,或P<0.01);Bre组、Cis组以及Bre+Cis组对黑色素瘤的细胞凋亡率分别为(13.78±1.66)%,(12.90±1.10)%,(18.74±1.22)%,与对照组(2.02±0.32)%相比差异有统计学意义(P<0.01)。结论 Bre、Cis通过影响细胞周期以及血管形成,从而抑制小鼠黑色素瘤的生长。
Objective To study the effect of breviscapine (Bre), cisplatin (Cis) and their combination on melanoma in mice and its mechanism. Methods C57 mice were used to establish subcutaneous xenograft model. The mice were divided into 4 groups: control group (DMSO), Bre group (4μg · g ~ (-1) Bre), Cis group g ~ (-1) Cis). Bre + Cis group (Bre and Cis injection) were injected every other day for 15 days. The tumor volume was measured after each treatment. The expression of Brd U and CD31 was detected by immunohistochemistry. The tumor cell cycle was detected by flow cytometry. Mouse melanoma cell proliferation was measured by 3- (4,5-dimethylthiazol-2) -2,5-diphenyltetrazolium bromide (MTT) method. Results Compared with the control group, the tumor volume and tumor weight of Bre group, Cis group and Bre + Cis group were significantly decreased (P <0.05, P <0.01 or P <0.01). At the same time, all three groups could significantly inhibit the melanoma cells (13.78 ± 1.66)%, (12.90 ± 1.10)%, (12.90 ± 1.10)% respectively in Bre, Cis and Bre + Cis groups (P <0.05 or P <0.01) 18.74 ± 1.22)%, which was significantly different from that of the control group (2.02 ± 0.32)% (P <0.01). Conclusion Bre and Cis inhibit the growth of mouse melanoma by affecting cell cycle and angiogenesis.