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丹参水溶性提出物发现有抑制体外环腺苷酸磷酸二酯酶(cAPD)的作用。这种抑制对被试动物和组织具有普遍性,但以家兔子宫、大脑、膀胱和肺中的cAPD最敏感。每个测定含微克量级的丹参水溶性有机物即可产生明显抑制作用,剂量增加到1～2毫克出现完全抑制。兔脑匀浆上清液中的蛋白质对其cAPD有保护作用。酶作用动力学的研究指出,丹参水溶性成分对兔脑cAPD表现典型的非竞争性抑制,并且是不可逆性抑制。丹参水溶性提出物用硅胶G薄板层析可检出16个斑点。用有机溶剂分离的部分4(主要为斑点13)对兔脑cAPD的抑制力最强,其50%抑制量为8微克/毫升,比茶碱强32倍。丹参水溶性成分可能通过抑制cAPD而发挥某些生理效应。
The water-soluble extract of Salvia miltiorrhiza has been found to inhibit the action of cyclic adenylate phosphodiesterase (cAPD) in vitro. This inhibition was universal for the animals and tissues tested, but the cAPD was most sensitive in the rabbit house, brain, bladder, and lung. Each determination containing micrograms of water-soluble organic compounds of Salvia miltiorrhiza can produce a significant inhibitory effect, the dose increased to 1-2 mg completely inhibited. The protein in rabbit brain homogenate had protective effect on its cAPD. The study of the kinetics of enzyme action indicated that the water-soluble constituents of Salvia miltiorrhiza show a non-competitive inhibition of rabbit cAPD and are irreversibly inhibited. The water soluble extract of Salvia miltiorrhiza can be used to detect 16 spots using silica gel G thin-layer chromatography. Fraction 4 (mainly spot 13) isolated with organic solvents had the strongest inhibitory effect on cAPD in rabbit brains, with a 50% inhibition of 8 μg/ml, 32 times stronger than theophylline. Salvia miltiorrhiza water-soluble ingredients may exert some physiological effects by inhibiting cAPD.