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目的探讨移植巨噬细胞对大鼠缺血再灌注引起的心肌梗死后细胞外基质修复和心室重塑的影响。方法贴壁法培养Wistar大鼠腹腔巨噬细胞,建立Wistar大鼠心肌缺血-再灌注模型,随机分为对照组(AMI组,n=23),巨噬细胞移植组(MΦ组,n=20),假手术组(n=12)。术后第7天和第28天,取大鼠左室心肌组织进行组织病理学分析,分别测定膨展指数、梗死面积、非梗死区心肌细胞横截面积(CSA)、梗死区及非梗死区胶原容积分数(CVF)、梗死区胶原成熟程度。结果与AMI组比较,MΦ组术后第7天膨展指数[(0.27±0.13)vs(0.41±0.19),P<0.05]、非梗死区CSA[(330.65±57.30)μm2vs(408.00±99.98)μm2,P<0.01]均显著减少,MΦ组术后第28天膨展指数[(0.30±0.11)vs(0.48±0.12,P<0.01]、非梗死区CSA[(335.80±92.52)μm2 vs(515.41±93.13)μm2,P<0.01]也均显著减少;术后第7天和第28天MΦ组梗死区CVF显著大于AMI组[(53.51±5.82)%vs(45.05±3.66)%,(79.17±5.58)%vs(73.17±5.94)%,P<0.01],非梗死区CVF显著小于AMI组[(2.37±0.65)%vs(4.84±1.81)%,(2.64±0.63)%vs(6.01±1.34)%,P<0.01],梗死区胶原成熟程度显著大于AMI组[(0.566±0.009)vs(0.509±0.011),(2.162±0.047)vs(1.454±0.020),P<0.01]。结论巨噬细胞心肌内移植促进梗死区胶原沉积和成熟度增加,减少梗死范围,减轻非梗死区纤维化程度和心肌肥厚程度,从而改善心肌梗死后左室重塑。
Objective To investigate the effects of transplanted macrophages on extracellular matrix remodeling and ventricular remodeling after myocardial ischemia-reperfusion in rats. Methods Wistar rat peritoneal macrophages were cultured by adherence method. Myocardial ischemia - reperfusion model was established in Wistar rats and randomly divided into control group (AMI group, n = 23), macrophage transplantation group (MΦ group, n = 20), sham operation group (n = 12). On the 7th day and the 28th day after operation, the left ventricular tissue of the rats was taken for histopathological analysis. The swelling index, infarction area, CSA, infarct area and non-infarct area Collagen volume fraction (CVF), degree of collagen maturation zone. Results Compared with AMI group, the swelling index of the MΦ group on the 7th postoperative day was (0.27 ± 0.13) vs (0.41 ± 0.19), P <0.05, and the non-infarct area CSA was (330.65 ± 57.30) μm2 vs (408.00 ± 99.98) μm2, P <0.01]. The swelling index in the MΦ group on day 28 [(0.30 ± 0.11) vs (0.48 ± 0.12, P <0.01) and non-infarct area CSA [(335.80 ± 92.52) 515.41 ± 93.13μm2, P <0.01]. The CVF in the MΦ group was significantly higher than that in the AMI group [(53.51 ± 5.82)% vs (45.05 ± 3.66)%] on the 7th and 28th day after surgery (2.37 ± 0.65)% vs (4.84 ± 1.81)%, (2.64 ± 0.63)% vs (6.01 ± ± 2.81)% vs (73.17 ± 5.94)%, P <0.01] 1.34)%, P <0.01]. The degree of collagen maturation in infarcted area was significantly higher than that in AMI group [(0.566 ± 0.009) vs (0.509 ± 0.011) vs (2.162 ± 0.047 vs 1.454 ± 0.020, P <0.01] Intramyocardial transplantation of macrophages promotes collagen deposition and maturity in the infarct zone, decreases infarct size, reduces fibrosis and myocardial hypertrophy in non-infarcted areas, and improves left ventricular remodeling after myocardial infarction.