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目的:探讨酸性成纤维细胞生长因子(aFGF)对大鼠心脏缺氧/再灌损伤的保护作用及作用机制。方法:在Langendorff装置上建立心脏缺氧/再灌模型,用BL-410生物机能实验系统记录经aFGF预处理的大鼠心脏缺氧/再灌前后左室发展压(LVDP)、左室内压上升/下降的最大、最小速率((dp/dt)m ax、(dp/dt)m in)的变化,测定不同时点冠脉流出液中的乳酸脱氢酶(LDH)、丙二醛(MDA)、超氧化物歧化酶(SOD)含量。结果:aFGF预处理可促进心脏缺氧/再灌后心功能恢复,逆转缺氧/再灌导致的SOD降低和LDH、MDA升高。结论:aFGF对缺氧/再灌损伤心脏具有一定的保护作用,其作用机制可能与提高自由基清除酶活性及抑制脂质过氧化有关。
Objective: To investigate the protective effect and mechanism of acidic fibroblast growth factor (aFGF) on rat cardiac anoxia / reperfusion injury. Methods: Cardiac hypoxia / reperfusion model was established on Langendorff apparatus. Before and after hypoxia / reperfusion, atrial fibrillation (LVDP) and left ventricular pressure (Dp / dt) m ax, (dp / dt) m in) at different time points were measured and the levels of LDH, MDA ), Superoxide dismutase (SOD) content. Results: aFGF preconditioning could promote cardiac function recovery after hypoxia / reperfusion and reverse the decrease of SOD and the increase of LDH and MDA caused by hypoxia / reperfusion. CONCLUSION: aFGF can protect heart from hypoxia / reperfusion injury. Its mechanism may be related to the increase of free radical scavenging enzyme activity and inhibition of lipid peroxidation.