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目的 探讨中国人头颈部鳞状细胞癌 (SCCHN)多染色体上等位基因的杂合性丢失 (L OH)和微卫星不稳定 (MI)的频率。方法 采用聚合酶链反应结合二核苷酸的重复序列多态性方法 ,选取 6对染色体 (3p,4q,7q,9p,17p和 18q)上 18个微卫星位点对 30例 SCCHN手术配对组织进行分析。结果 30例 SCCHN组织中有 12例 (4 0 % )组织至少有一个微卫星位点发生 L OH,多数位点 L OH的频率普遍比已报道的文献低。有 7个位点(D7S480、D7S5 2 2、D9S162、D9S168、D9S30 4、D9S171和 D17S5 2 0 )具有统计学意义 (P<0 .0 5 ) ,然而这些位点的L OH与肿瘤病理学类型、肿瘤大小及转移性无显著相关性。此外 ,MI仅在 4例患者中出现 ,没有 1例患者符合 MI的判定标准 ,即需两个或两个以上的微卫星多态位点的异常。结论 主要发生在 D7S480、D7S5 2 2、D9S162、D9S168、D9S30 4、D9S171和 D17S5 2 0位点的 L OH可能存在与部分 SCCHN有关的潜在的肿瘤抑制基因 ,而微卫星不稳定性与 SCCHN发生的关系不大
Objective To investigate the frequency of loss of heterozygosity (L OH) and microsatellite instability (MI) on polychromosomal alleles in Chinese head and neck squamous cell carcinoma (SCCHN). METHODS: Using the polymerase chain reaction and dinucleotide repeat sequence polymorphism method, 18 microsatellite loci on 6 pairs of chromosomes (3p, 4q, 7q, 9p, 17p, and 18q) were selected and 30 pairs of SCCHN surgical pairing tissues were selected. Conduct the analysis. Results LOH was found in at least one microsatellite locus in 12 cases (40%) of the 30 SCCHN tissues. The frequency of LOH at most loci was generally lower than that reported in the literature. There were 7 sites (D7S480, D7S52, D9S162, D9S168, D9S304, D9S171, and D17S502) statistically significant (P < 0.05), however, the LOH of these sites and tumor pathology type There was no significant correlation between tumor size and metastasis. In addition, MI occurred only in 4 patients and none of the patients met the MI criteria, ie, two or more microsatellite polymorphism sites were abnormal. Conclusions The LOHs that mainly occur in D7S480, D7S52, D9S162, D9S168, D9S304, D9S171 and D17S5 20 sites may have potential tumor suppressor genes related to some SCCHNs, but microsatellite instability and SCCHN occur. Little relationship