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目的以芹菜素和芹菜素-7-O-β-D-(-6“-p-香豆酰基)-葡萄糖苷为指标性成分,建立蒙药材细叶铁线莲的薄层鉴别和含量测定方法。方法薄层鉴别采用硅胶G薄层板,以氯仿-甲醇-甲酸(4∶0.5∶0.2)为展开剂,3%三氯化铝乙醇溶液显色,置紫外灯(365nm)下检视。含量测定采用ODS填充的色谱柱,乙腈-2%醋酸水溶液(30∶70)为流动相,335nm下测定。结果薄层色谱方法斑点清晰,专属性强。HPLC含量测定法,芹菜素和芹菜素-7-O-β-D-(-6”-p-香豆酰基)-葡萄糖苷均在0.05~1.60μg线性良好(r=0.9999),平均回收率分别为100.3%(RSD为0.33%)和100.5%(RSD为0.29%),在6批次药材中的含量分别为0.181~0.368 mg·g-1和0.819~2.030 mg·g-1。结论此方法可有效鉴别细叶铁线莲,准确测定药材中芹菜素和芹菜素-7-O-β-D-(-6“-p-香豆酰基)-葡萄糖苷的含量,为该药材的质量控制提供方法和依据。
OBJECTIVE To establish a thin layer identification and content determination of Clematis sylvestris L. using Apigenin and apigenin-7-O-β-D - (- 6 ”-p-coumaroyl) -glucoside as an index component Methods Thin layer identification was performed on silica gel TLC plate using chloroform-methanol-formic acid (4:0.5:0.2) as developing solvent and 3% aluminum trichloride ethanol solution for color development under UV light (365 nm) The determination of content was carried out by column packed with ODS and acetonitrile-2% acetic acid aqueous solution (30:70) as the mobile phase at 335nm.Results The method of TLC was clear and specific.HPLC determination, apigenin and celery The average recoveries of all the compounds were linear at 0.05-1.60 μg (r = 0.9999) and the average recoveries were 100.3% (RSD 0.33 %) And 100.5% (RSD 0.29%), respectively, and the contents in six batches were 0.181 ~ 0.368 mg · g-1 and 0.819 ~ 2.030 mg · g-1, respectively. Conclusion This method can effectively identify Clematis spp. And accurately determine the content of apigenin and apigenin-7-O-β-D - (- 6 "-p-coumaroyl) Quality control of herbs provide the basis and method.