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肝细胞坏死是病毒性肝炎病变特点上一,但其坏死机理尚未阐明。为此,本文以气球样肝细胞为重点,用电镜、组化及电镜细胞化学等方法观察SAH、CAH、CPH及GalN致大鼠肝细胞损伤。结果显示气球样肝细胞主要表现为线粒体肿胀,内质网扩张,胞质局灶性溶解及质膜受损,伴相应酶活性下降,包括Na~+-K~+-ATP酶、G-6-P酶、SDH等。由于质膜损伤,Na~+-K~+-ATP酶活性下降,可导致细胞内Ca~(2+)浓度增加,后者激活磷脂酶等,严重破坏膜结构,致使细胞死亡。本文用ARS染色证实了气球样细胞Ca~(2+)浓度增加,提示病毒性肝炎细胞坏死可能与Na~+-K~+-ATP酶活性下降,细胞内Ca~(2+)浓度增加有关
Hepatocyte necrosis is a feature of viral hepatitis, but its necrotic mechanism has not been elucidated yet. To this end, we focus on balloon-like hepatocytes and observe the injury of hepatocytes induced by SAH, CAH, CPH and GalN by electron microscopy, histochemistry and electron microscopy cytochemistry. The results showed that the majority of balloon-like hepatocytes were mitochondria swelling, endoplasmic reticulum dilatation, focal cytoplasmic lysis and plasma membrane damage, with associated enzyme activity decreased, including Na ~ + -K ~ + -ATPase, G-6 -P enzyme, SDH and so on. Due to the plasma membrane damage, Na ~ + -K ~ + -ATPase activity decreased, can lead to increased intracellular Ca ~ (2+) concentration, which activates phospholipase, severely damage the membrane structure, resulting in cell death. ARS staining confirmed that the balloon-like cells Ca 2+ concentration increased, suggesting that viral hepatitis necrosis may be Na ~ + -K ~ + -ATPase activity decreased intracellular Ca ~ (2+) concentration increased