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在细胞融合技术中,使原生质体高频率地再生是其中至关重要的一环。然而,在食用菌的细胞融合研究中,诸如已报道的香菇、平菇、草菇等,其原生质体的再生率均较低,一般在10～1%之间,有的甚至在1%以下,这就给进一步工作带来一定困难。造成原生质体再生率低下的原因,可能是由原生质体自身质量所引起的。已有报道证实,平菇原生质体有36%的细胞无核。我们认为,更主要的原因可能还在于对原生质体再生的条件研究不够。目前还未见有关再生条件的研究报告。我们在成功地制备黑木耳菌丝原生质体的基础上,对其再生的某些条件进行了初步的研究。
In cell fusion technology, the regeneration of protoplasts at high frequency is one of the most important links. However, in the researches of cell fusion of edible fungi, such as the reported mushrooms, mushrooms, mushrooms, etc., the regeneration rate of protoplasts is low, generally between 10 and 1%, and some even below 1% , Which will bring some difficulties to further work. Reasons for the low protoplast regeneration rate may be caused by the protoplast’s own quality. It has been reported that Pleurotus ostreatus protoplasts 36% of the cells without nucleus. In our opinion, the more important reason may lie in the fact that there are not enough studies on the conditions of protoplast regeneration. No research report on regeneration conditions is available yet. Based on the successful preparation of the black fungus mycelium protoplasts, we carried out a preliminary study on some conditions for its regeneration.