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目的 :探讨抑癌基因p53突变和人类乳头状瘤病毒 16,18型 (HPV16,18)感染与喉鳞癌的关系及二者在喉鳞癌中的相关性。方法 :应用聚合酶链反应结合单链构象多态性分析 (PCR -SSCP)技术对4 0例喉鳞癌组织中HPV16,18DNA及p53基因第 5,7外显子的突变进行检测。结果 :HPV16,18的检出率分别为 35% ( 14/ 4 0 )和 10 % ( 4 / 4 0 )。 12例 ( 30 % )标本p53基因突变 ,其中第 5外显子 10例( 2 5% ) ,第 7外显子 2例 ( 5% ) ,p53基因在HPV阳性和阴性标本中的突变率分别为 33 3%和 2 7 3% ,二者差别无显著性 ( χ2 =0 1732 ,P >0 0 5)。结论 :HPV感染和p53基因突变在喉鳞癌的发生发展过程中都起重要作用 ,但二者之间无相关性
Objective: To investigate the relationship between the mutation of p53 tumor suppressor gene and human papillomavirus type 16 and HPV16 and laryngeal squamous cell carcinoma (LSCC) and their relationship in laryngeal squamous cell carcinoma. Methods: The mutations of HPV16, 18 DNA and p53 exons 5 and 7 in 40 cases of laryngeal squamous cell carcinoma were detected by polymerase chain reaction and single strand conformation polymorphism (PCR-SSCP). Results: The detection rates of HPV16 and HPV18 were 35% (14/40) and 10% (4/40) respectively. The mutation of p53 gene was found in 12 cases (30%) specimens, including 10 cases (2.5%) of exon 5 and 2 cases (7%) of exon 7, and the mutation rate of p53 gene in HPV positive and negative specimens respectively 33 3% and 273% respectively. There was no significant difference between them (χ2 = 0 1732, P> 0.05). Conclusion: HPV infection and p53 gene mutation play an important role in the development of laryngeal squamous cell carcinoma, but there is no correlation between the two