论文部分内容阅读
目的 动态观察小于胎龄(ＳＧＡ)鼠肝脏组织胰岛素样生长因于－Ⅰ(ＩＧＦ－Ⅰ)及ＩＧＦ结合蛋白(ＩＧＦＢＰｓ)的基因表达变化,以探讨ＳＧＡ的可能发病机制。方法 子宫动脉结扎(ＵＡＬ)建立宫内发育迟缓(ＩＵＧＲ)模型,应用逆转录(ＲＴ)－ＰＣＲ测定ＳＧＡ鼠生后第１、３、７、１４、２１天肝脏组织ＩＧＦ－Ⅰ、ＩＧＦＢＰｓ ｍＲＮＡ表达变化,并与正常组比较。结果 (１)正常新生鼠生后肝脏组织 ＩＧＦ－ⅠｍＲＮＡ水平逐渐上升,而 ＩＧＦＢＰ１、ＩＧＦＢＰ２ ｍＲＮＡ水平无明显改变。(２)轻度 ＳＧＡ组出生时仅 ＩＧＦ－ＢＰ１ｍＲＮＡ水平上升,生后逐渐下降,至生后 １４天达到正常组水平,而 ＩＧＦ－Ⅰ、ＩＧＦＢＰ２ ｍＲＮＡ水平与对照组比较无明显差异。(３)重度ＳＧＡ组出生时ＩＧＦ－ⅠｍＲＮＡ水平下降,生后则逐渐上升,至生后２１天仍低于正常组水平;ＩＧＦＢＰ１、ＩＧＦＢＰ２ ｍＲＮＡ水平出生时升高,生后则逐渐下降,至生后 ２１天仍高于正常组水平。结论 轻度 ＳＧＡ存在一过性高 ＩＧＦＢＰ１ｍＲＮＡ水平,重度 ＳＧＡ持续存在低ＩＧＦ－ⅠｍＲＮＡ、高 ＩＧＦＢＰ１、ＩＧＦＢＰ２ ｍＲＮＡ水平,其不良的宫内外生长可能与此有关。
Objective To observe the gene expression changes of insulin-like growth factor (IGF-Ⅰ) and IGF-binding protein (IGFBPs) in liver of small-gestational age (SGA) rats to investigate the possible pathogenesis of SGA. Methods Intrauterine growth retardation (IUGR) model was established by uterine artery ligation (UAL). Reverse transcription (RT) -PCR was used to detect the mRNA expression of IGF-Ⅰ and IGFBPs in the liver on day 1,3,7,14,21 Change, and compared with the normal group. Results (1) Normal neonatal rat liver tissue IGF-ⅠmRNA levels gradually increased, while IGFBP1, IGFBP2 mRNA levels did not change significantly. (2) The level of IGF-BP1mRNA in the mild SGA group was only increased at birth, decreased gradually after birth, and reached the level of normal group on the 14th day after birth. There was no significant difference between IGF-Ⅰ and IGFBP2mRNA levels in control group. (3) The levels of IGF-ⅠmRNA in the severe SGA group decreased at birth, but gradually increased after birth, and still remained lower than those in the normal group at 21 days after birth. The levels of IGFBP1 and IGFBP2 mRNA increased at birth and gradually decreased after birth After 21 days still higher than the normal group level. Conclusions There is a transiently high level of IGFBP1mRNA in mild SGA, low level of IGF-ⅠmRNA, high level of IGFBP1 and IGFBP2mRNA in severe SGA, and its abnormal intrauterine and extrauterine growth may be related to it.