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采用液相色谱-三重四级杆复合线性离子阱质谱(HPLC/Q-TRAP MS),在正离子检测模式下,对鲤鱼(Cyprinus carpio)腹腔注射恩诺沙星给药后肝脏中的代谢产物进行分析。根据保留时间和各色谱峰质谱裂解规律,推测了恩诺沙星在鲤鱼肝脏中的代谢产物,同时,根据二级质谱碎片离子推测代谢途径及代谢产物结构。结果显示,恩诺沙星进入鲤鱼肝脏后,除以恩诺沙星原形药物(M0)和N-去乙基代谢产物环丙沙星(M2)形式存在外,还有少量恩诺沙星脱羧代谢产物(M1)和恩诺沙星羟基化代谢产物(M3-1和M3-2)。该研究可为深入了解恩诺沙星在水生动物体内代谢产物及代谢机理提供理论基础,为在水产养殖生产中科学、合理地使用恩诺沙星提供参考。
In the positive ion detection mode, the metabolites of enrofloxacin in the liver after intraperitoneal administration of enrofloxacin (Cyprinus carpio) were analyzed by liquid chromatography-triple quadrupole linear ion trap mass spectrometry (HPLC / Q-TRAP MS) Analyze. The metabolites of enrofloxacin in liver of carp were speculated according to the retention time and the cleavage rules of chromatographic peaks. Meanwhile, the metabolic pathway and the structure of metabolites were speculated based on the secondary ion mass spectrometry fragment ions. The results showed that enrofloxacin entered the carp liver, in addition to the enrofloxacin prototype drug (M0) and N-ethyl metabolites ciprofloxacin (M2) in the form of existence, there are a small amount of enrofloxacin decarboxylation Metabolites (M1) and enrofloxacin hydroxylated metabolites (M3-1 and M3-2). This study may provide a theoretical basis for further understanding of enrofloxacin metabolites and metabolic mechanisms in aquatic animals and provide a reference for the scientific and rational use of enrofloxacin in aquaculture production.