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为研究大肠杆菌Escherichia coli侵染引发亚洲玉米螟Ostrinia furnacalis Guenée幼虫免疫应激反应的机理,本实验测定了分别注射生理盐水以及3×103,3×104,3×105和3×106个细胞/mL大肠杆菌后亚洲玉米螟5龄幼虫血淋巴中血细胞总数(THC)、颗粒细胞和浆血细胞数量,血清中酚氧化酶(PO)、谷胱甘肽过氧化物酶(GSH-px)、谷胱甘肽还原酶(GR)和谷胱甘肽-S-转移酶(GST)的活性,通过流式细胞仪分析了血细胞活性氧自由基(ROS)水平的动态变化。结果表明:与对照组相比,注射3×105和3×106个细胞/mL大肠杆菌细胞后12h,可引起亚洲玉米螟5龄幼虫THC及浆血细胞、颗粒细胞数量明显上升(P<0.01),同时应激产生大量ROS。3×104,3×105和3×106个细胞/mL大肠杆菌3个不同浓度处理组均引起幼虫体内PO活性显著升高(P<0.01),诱导幼虫血清中GSH-px,GST及GR的活性上升(P<0.01)。这些结果表明,亚洲玉米螟幼虫受到大肠杆菌侵染后,其血淋巴细胞免疫和体液免疫能力受到显著影响,可诱导血清中GSH-px,GST和GR活性升高以清除过多的ROS,防止其毒害。
In order to study the mechanism of immune stress induced by Escherichia coli infection in Ostrinia furnacalis Guenée larvae, the effects of saline injection and 3 × 103, 3 × 104, 3 × 105 and 3 × 106 cells / (THC), the number of granulosa cells and plasma cells, the levels of phenoloxidase (PO), glutathione peroxidase (GSH-px) and glutathione peroxidase (GSH-px) in the 5th instar larvae of E.coli post- The activities of glutathione reductase (GR) and glutathione-S-transferase (GST) were assayed by flow cytometry to analyze the dynamic changes of reactive oxygen species (ROS) levels in blood cells. The results showed that the number of THC, plasma cells and granulosa cells of 5th instar larvae of Asiaticocular borer were significantly increased (P <0.01) after injection of 3 × 105 and 3 × 106 cells / mL E.coli cells, , While stress generated a lot of ROS. The activities of PO in the larvae were significantly increased at 3 × 104, 3 × 105 and 3 × 106 cells / mL of E. coli at 3 different concentrations (P <0.01), and the levels of GSH-px, GST and GR Activity increased (P <0.01). These results indicate that Asian corn borer larvae infected with Escherichia coli have a significant effect on their hemolymph and humoral immunity and can induce elevated serum GSH-px, GST and GR activity to clear excess ROS and prevent It’s poisoned.