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目的探讨HBV DNA定量PCR检测对临床应用复肝宁联合香菇多糖片联合治疗慢性无症状HBsAg(+)携带者(ASC)的临床指导意义。方法采用Light Cycler实时荧光定量PCR仪对门诊82例患者进行血清HBV DNA检测;分别观察用药后6个月和12个月的HBV DNA结果并进行统计学分析。结果治疗后6个月和12个月的有效率分别为19.51%、36.59%;治疗6个月与治疗前对比差异有统计学意义(P<0.01);治疗12个月与治疗前HBV DNA阳性率对比差异更加显著;治疗12个月与治疗6个月后HBV DNA阳性率对比差异也有统计学意义(P<0.01)。结论复肝宁联合香菇多糖片治疗ASC有明显疗效,其有效率随着用药时间的延长而增高,HBV DNA定量检测灵敏度高、重现性好的特点为临床提供了良好的疗效指标。
Objective To investigate the clinical significance of HBV DNA quantitative PCR in the clinical treatment of chronic asymptomatic HBsAg (+) carriers (ASC) in combination with Fuganning combined with lentinan. Methods Serum HBV DNA was detected in 82 outpatients by Light Cycler real-time PCR. The HBV DNA levels at 6 months and 12 months after treatment were observed and statistically analyzed. Results The effective rates at 6 months and 12 months after treatment were 19.51% and 36.59% respectively. The difference between the 6-month and 6-month treatment was statistically significant (P <0.01) The differences between the two groups were statistically significant (P <0.01). There was also significant difference in the positive rate of HBV DNA between the 12-month treatment and 6-month treatment (P <0.01). Conclusion Fructus Qu Gan combined with lentinan treatment of ASC has a significant effect, the effective rate increased with the extension of medication time, HBV DNA quantitative detection of high sensitivity and good reproducibility of the clinical provides a good indicator of efficacy.