近年来分子靶向治疗在非小细胞肺癌中备受关注,其中EGFR基因状态是决定是否用EGFR-TKI治疗的先决条件。目前最常用于EGFR检测的方法是ARMS法,该方法灵敏度高、时间短、操作简单,易于在临床样品中检测开展。但由于标本因素的影响、样本DNA抽提、假阳性、假阴性、加样误差 In recent years, molecular targeted therapy has drawn much attention in non-small cell lung cancer. The status of EGFR gene is a prerequisite for the treatment of EGFR-TKI. At present, the most commonly used method for EGFR detection is the ARMS method, which has the advantages of high sensitivity, short time, simple operation and easy detection in clinical samples. However, due to the influence of specimen factors, sample DNA extraction, false positive, false negative, add error