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在ShimpakCLC-ODS柱上,甲醇-0.02mol/L醋酸钠溶液(62:38)为流动相,高效液相色谱法同时测定活络灵胶囊中泼尼松龙和吲哚美辛。紫外检测波长254nm。二组分线性关系良好,6次测定的平均回收率±RSD%分别为100.1%±0.04%,996%±0.22%。本法用于制剂的含量及含量均匀度测定,切实可行.
Methanol-0.02 mol / L sodium acetate solution (62:38) was used as the mobile phase on a Shimpak CLC-ODS column for the simultaneous determination of prednisolone and indomethacin by HPLC. UV detection wavelength 254nm. The linear relationship between the two components was good. The average recovery of six determinations was ± 100% ± 0.04% and ± 99% ± 0.22%, respectively. This method for the determination of content and content uniformity, feasible.