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本文以ｒＩＬ6的纯化为例进行实验。用超声破碎的方法提取出菌体蛋白粗提物和非相关包涵体杂质蛋白(ｒＩＬ2包涵体),进而用常规免疫法制备兔抗菌体蛋白和包涵体杂质蛋白抗血清。从抗血清中用亲和层析法分离出抗菌体蛋白和包涵体杂质蛋白的特异ＩｇＧ,然后偶联ＣＮＢｒ激活的Ｓ
In this paper, the purification of rIL6 as an example for experiments. The bacterial protein crude extract and unrelated inclusion protein (rIL2 inclusion body) were extracted by sonication, and then the rabbit anti-bacterial protein and the inclusion protein anti-serum were prepared by routine immunization. The specific IgG of the antimicrobial protein and inclusion body impurity protein was separated from the antiserum by affinity chromatography and then the CNBr-activated S