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目的：为深入揭示乙型肝炎病毒（ＨＢＶ）宫内感染及其危险因素。方法：采用多聚酶链反应（ＰＣＲ）技术结合酶联免疫吸附法（ＥＬＩＳＡ）对广西１１６例ＨＢｓＡｇ阳性和５２例ＨＢｓＡｇ阴性孕妇外周血及其引产胎儿胎心血进行ＨＢＶ血清学标志物和ＨＢＶＤＮＡ检测。结果：ＨＢｓＡｇ阳性、ＨＢｓＡｇ和ＨＢｅＡｇ双阳性、ＨＢＶＤＮＡ阳性孕妇胎儿ＨＢＶＤＮＡ检出率（５１．３％、９０．２％、８３．８％）均分别显著高于其对照组（３．９％、２１．２％、０．０％）。Ｌｏｇｉｓｔｉｃ回归分析选出的孕妇ＨＢＶ宫内感染危险因素有ＨＢｓＡｇ、ＨＢｅＡｇ、ＨＢＶＤＮＡ和孕期。结论：广西ＨＢＶ宫内感染率较高，ＨＢｓＡｇ阴性孕妇也可能发生ＨＢＶ宫内感染；孕妇ＨＢｓＡｇ、ＨＢｅＡｇ尤其是ＨＢＶＤＮＡ阳性以及孕期是ＨＢＶ宫内感染的主要危险因素。
Objective: To deeply reveal the intrauterine infection of hepatitis B virus and its risk factors. Methods: HBV serological markers and HBVDNA were detected by polymerase chain reaction (PCR) combined with enzyme-linked immunosorbent assay (ELISA) in peripheral blood of 116 HBsAg-positive and 52 HBsAg-negative pregnant women in Guangxi. Results: The positive rates of HBVDNA in HBsAg-positive, HBsAg-positive and HBeAg-positive, HBVDNA-positive fetuses were significantly higher than those in the control group (3.9%, 21%, 51.3%, 90.2%, 83.8% .2%, 0.0%). Logistic regression analysis of selected pregnant women with intrauterine HBV infection risk factors HBsAg, HBeAg, HBVDNA and during pregnancy. Conclusion: The intrauterine infection rate of HBV in Guangxi is high, but HBsAg-negative pregnant women may also have intrauterine infection of HBV. Pregnant women with positive HBsAg, HBeAg, especially HBVDNA and pregnant women are the main risk factors of HBV intrauterine infection.