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目的观察柠檬提取物对细菌生物膜的消除作用,对柠檬提取物抑制多重耐药金黄色葡萄球菌的机制进行初步研究,以及对环境中富集的病毒消除作用的研究。方法采用高分子滤膜法制备金黄色葡萄球菌生物膜后,加入柠檬提取物分别作用1、2和3 h,通过细菌菌落计数判定不同生物膜的细菌存活数;采用SDS-PAGE电泳法,观察在柠檬提取物作用下菌体蛋白质的变化;EcoR I酶切金黄色葡萄球菌DNA,观察酶切图谱的改变;在低渗环境下动态观察柠檬提取物在不同作用时间对细菌细胞壁的影响;通过柠檬提取物对病毒作用后RNA量的变化,说明柠檬提取物具有杀灭病毒的作用。结果柠檬提取物对生物膜中的细菌具有杀菌作用,且随作用时间的延长而逐渐增强;柠檬提取物对细菌蛋白质的组成和表达量均有一定影响,同时细菌DNA的EcoR I酶切图谱发生改变;柠檬提取物作用15 min时细菌呈现明显的胞壁缺损现象,即细菌个体胀大,呈现大球形;经柠檬提取物作用后,RNA病毒被杀灭。结论结果显示柠檬提取物对耐药金黄色葡萄球菌所形成的生物膜具有明显抑制作用;在柠檬提取物作用下,菌体蛋白合成量发生改变,同时对细菌的DNA和细胞壁有明显的影响。柠檬提取物处理病毒悬液后,病毒RNA量显著减少,说明病毒RNA结构被破坏。
OBJECTIVE To observe the elimination effect of lemon extract on bacterial biofilm, and to study the mechanism of lemon extract inhibiting multidrug-resistant Staphylococcus aureus and its role in eliminating virus enriched in the environment. Methods Staphylococcus aureus biofilm was prepared by macromolecule membrane filtration method. After lemon extract was added for 1, 2 and 3 h respectively, the number of bacteria in different biofilms was determined by bacterial colony counting. SDS-PAGE electrophoresis was used to observe Leymus extract under the action of bacterial protein changes; EcoR I Staphylococcus aureus DNA, observed changes in digestion patterns; dynamic observation of lemon extract in the hypotonic environment at different times on the impact of bacterial cell wall; by Lemon extract on the amount of RNA after the virus changes, indicating that the lemon extract has the role of killing the virus. Results The lemon extract had a bactericidal effect on the bacteria in the biofilm, and gradually increased with the prolongation of time. The lemon extract had some influence on the composition and expression of the bacterial protein, and the EcoR I digestion map of the bacterial DNA Change; lemon extract 15 min when the bacteria showed obvious cell wall defects, namely, the individual bacteria swollen, showing a large spherical; lemon extract by the RNA virus was killed. Conclusion The results showed that lemon extract inhibited the formation of biofilm in S. aureus. Under the action of lemon extract, the amount of bacterial protein synthesis changed, while the bacterial DNA and cell wall had obvious effects. After the lemon extract was treated with the virus suspension, the amount of viral RNA was significantly reduced, indicating that the viral RNA structure was destroyed.