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目的:改良卷叶贝母现有品种,增加其药用成分含量,丰富遗传育种资源。方法:本研究通过使用低温沙藏层积处理卷叶贝母种子,使其完成生理和形态后熟,再采用不同浓度的秋水仙素溶液对卷叶贝母种子进行不同时间的诱导处理。结果:经植物形态学和染色体数检测,诱导后的材料具有明显的多倍体特征。结论:卷叶贝母种子用30 mg/L的GA3处理32 h,再经沙藏70 d,其种子萌发率为67.0%;沙藏处理成熟后的种子用0.1%的秋水仙素溶液处理48 h,其多倍体诱导率为85.7%。
OBJECTIVE: To improve the existing varieties of Fritillaria cirrhosa, increase the contents of its medicinal ingredients, and enrich genetic breeding resources. Methods: In this study, Fritillaria cirrhosa seeds were treated with low temperature sediment layers to make them physiologically and morphologically ripe, and different concentrations of colchicine solution were used to induce the seeds of Fritillaria cirrhosa at different times. Results: After the plant morphology and chromosome number detection, the induced material has obvious polyploidy characteristics. CONCLUSION: The seeds of Fritillaria cirrhosa were treated with 30 mg / L GA3 for 32 h, and the seed germination rate was 67.0% after 70 d of storage in the sand bank. The matured seed of the sand bank was treated with 0.1% colchicine solution for 48 h h, the polyploid induction rate was 85.7%.