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目的 :研究D5S818的遗传多态性及其法医学应用价值。方法 :应用聚合酶链式反应 (PCR)、聚丙烯酰胺凝胶电泳及银染显带技术对温州地区汉族 2 2 7名无关个体的D5S818位点进行分型 ,并检验D5S818基因型频率分布是否符合Hardy Weinberg平衡 ,计算法医学常用的各种概率 ,并与其他人群进行了比较。结果 :D5S818位点检出 8个等位基因、2 4种基因型。基因型频率的分布符合Hardy Weinberg平衡 (χ2 =12 .3,P >0 .5 ,df=16 ) ,观测杂合度h为 76 .6 5 +1.99% ,偶合机率为 0 .0 814 ,个体识别率为 0 .9186 ,多态信息含量为 0 .74 96 ,亲子关系指数为 2 .14 15 ,期望排除率为 0 .5 385。不同人群基因频率分布存在一定的差异。结论 :该研究所得到的等位基因频率数据可为温州汉族人群法医个体识别、亲子鉴定及遗传学研究提供更多依据。
Objective: To study the genetic polymorphism of D5S818 and its forensic value. Methods: Polymerase chain reaction (PCR), polyacrylamide gel electrophoresis and silver dyed banding technique were used to genotype D5S818 locus in 227 unrelated individuals in Han nationality in Wenzhou. The frequency distribution of D5S818 genotype was tested In line with the Hardy Weinberg equilibrium, the various probabilities commonly used in forensic science are calculated and compared to the rest of the population. Results: Eight alleles and 24 genotypes were detected at D5S818 locus. The distribution of genotype frequency was in accordance with Hardy Weinberg equilibrium (χ2 = 12.3, P> 0.05, df = 16). The observed heterozygosity h was 76.65 + 1.99% and the probability of coupling was 0.0814. Rate of 0. 9186, polymorphism information content of 0.74 96, parent-child relationship index of 2.14 15, expected exclusion rate of 0.5385. Gene frequency distribution of different populations there are some differences. Conclusion: The allele frequencies obtained from this study may provide more evidences for forensic identification, paternity testing and genetics research in Wenzhou Han population.