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目的研究4种水飞蓟素单体对鹅膏毒蕈中毒所致的肝损伤保护作用。方法选用60只大鼠,随机分为空白对照组、中毒模型组、水飞蓟宾组、异水飞蓟宾组、水飞蓟宁组及水飞蓟亭组6组(每组10只)。除空白对照组外,其余5组给予鹅膏毒蕈造模后,给予相应药物,3d后观察大鼠存活情况,测定肝功能相关指标、肝细胞膜流动性及肝细胞RNA聚合酶活性。结果与中毒模型组比较,4种水飞蓟素单体均能有效逆转鹅膏毒蕈中毒所致的肝功能指标异常,使之恢复正常(P<0.01);均能稳定肝细胞膜的流动性(P<0.01),同时能升高由于鹅膏毒蕈中毒引起的RNA聚合酶活性降低(P<0.01)。其中,水飞蓟宾的效果优于其余3种单体(P<0.05)。结论 4种水飞蓟素单体成分对鹅膏毒蕈所致肝损伤均具有保护作用,其中水飞蓟宾的活性最高;改善抗氧化活性、稳定膜流动性以及提高RNA聚合酶活性是其实现对大鼠肝损伤保护的作用机制之一。
Objective To study the protective effects of four kinds of silymarin on liver injury caused by poisoning of amanita. Methods Sixty rats were randomly divided into 6 groups (10 in each group): blank control group, poisoning model group, silybin group, isoproterenol group, silybin group and silymarin group, . Except for the blank control group, the other 5 groups were given Amanita phagocytosis, and the corresponding drugs were given. After 3 days, the survival of the rats was observed. The indexes of liver function, the fluidity of the liver membrane and the RNA polymerase activity of the hepatocytes were determined. Results Compared with the model group, all the four silymarin monomers could effectively reverse the abnormal liver function index caused by poisoning of amanita (P <0.01), all of which could stabilize the fluidity of the liver cell membrane (P < 0.01), and at the same time increased the RNA polymerase activity (P <0.01). Among them, silibinin was superior to the other three monomers (P <0.05). CONCLUSIONS Four kinds of silymarin monomer components have a protective effect on the liver injury induced by Amanita Toadstool, of which silibinin has the highest activity. Improving the antioxidant activity, stabilizing the membrane fluidity and increasing the RNA polymerase activity is the most important factor One of the mechanisms of rat liver injury protection.