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目的 观察异丙酚和硫喷妥钠对心肌细胞内游离Ｃａ２＋ 浓度的影响，以探讨静脉麻醉药对心肌细胞内游离Ｃａ２＋ 的作用及其机制。方法 心肌细胞分离培养７～９ 天，以Ｆｕｒａ２／ＡＭ 荧光指示剂负载后，实验组Ａ 加入实验用药孵育１０ 分钟进行Ｃａ２＋ 测定；实验组Ｂ：加入实验用药１０ 分钟后，加ＫＣｌ４０ｍ ｍ ｏｌ／Ｌ，１ 分钟后进行Ｃａ２＋ 测定。结果 临床剂量的异丙酚和硫喷妥钠对静息心肌细胞内Ｃａ２＋ 浓度无明显影响，超临床剂量的异丙酚和硫喷妥钠使静息细胞内Ｃａ２＋ 明显降低。４０ｍ ｍ ｏｌ／Ｌ的ＫＣｌ可使心肌细胞内Ｃａ２＋ 浓度明显升高，异丙酚和硫喷妥钠对高浓度ＫＣｌ所激发Ｃａ２＋ 内流均有明显的抑制，并呈剂量依赖性。结论 异丙酚和硫喷妥钠降低心肌细胞内钙离子浓度。他们在完整心肌的负性肌力作用可能与其部分抑制兴奋收缩耦联过程中钙离子内流和抑制静息状态下的钙溢流有关
Objective To observe the effects of propofol and thiopental on intracellular free Ca2 + concentration in cardiomyocytes in order to investigate the effect and mechanism of intravenous anesthetics on intracellular free Ca2 + in cardiomyocytes. Methods Cardiomyocytes were isolated and cultured for 7-9 days. After loading with Fura2 / AM fluorescent indicator, experimental group A was added with experimental drugs to incubate for 10 minutes for Ca2 + determination. In experimental group B, 10 minutes after adding experimental drugs, / L, 1 minute after Ca2 + determination. Results The clinical dose of propofol and thiopental had no significant effect on the intracellular Ca2 + concentration in resting cardiomyocytes. The superclinical doses of propofol and thiopental decreased the intracellular Ca2 + in resting cells significantly. KCl at a concentration of 40 m mol / L significantly increased the intracellular Ca2 + concentration in cardiomyocytes. Propofol and thiopental inhibited the Ca2 + influx induced by high concentrations of KCl in a dose-dependent manner. Conclusion Propofol and thiopental sodium can decrease the intracellular calcium concentration. Their negative inotropic effects on intact myocardium may be related to their partial inhibition of calcium influx and suppression of resting calcium status during excitatory and contractile coupling